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Pharmacia LKB Biotechnology Inc
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Biomol GmbH
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ONERA The French Aerospace Lab
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Wexler Surgical
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Alembic Instruments
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A-M Systems
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Image Search Results
Journal:
Article Title: Identification and comparative analysis of telomerase RNAs from Candida species reveal conservation of functional elements
doi: 10.1261/rna.1194009
Figure Lengend Snippet: Analysis of deduced telomeric repeats by Southern blot. (A) Chromosomal DNA samples from L. elongisporus CBS5301 (lane 1) and C. parapsilosis CBS2194 (lane 2), SR23 (lane 3), and CBS604T (lane 4) were separated by pulsed-field gel electrophoresis and subjected to Southern blot analysis using a 5′-end-labeled oligonucleotide consisting of three copies of the putative C. parapsilosis telomeric repeat as a probe (Supplemental Table S1). Ethidium bromide staining of this gel is shown on the left and the autoradiogram on the right. (B) Southern blot analysis of C. parapsilosis SR23 telomeric restriction fragments obtained by digestion of gDNA with AluI (lane 1), Csp6I (lane 2), HaeIII (lane 3), HinPI (lane 4), MseI (lane 5), Sau3A (lane 6), TaqI (lane 7), MspI (lane 8), AvaII (lane 9), and EcoRI (lane 10). Note that AvaII and MspI have a recognition site within the C. parapsilosis telomeric repeat. (C) Southern blot analysis of telomeric DNA fragments obtained by digestion of gDNA from C. metapsilosis MCO448T (lanes 1–4) and C. orthopsilosis MCO457T (lanes 5–8) with AluI (lanes 1,5), Csp6I (lanes 2,6), MspI (lanes 3,7), and Sau3A (lanes 4,8). 5′-end-labeled oligonucleotides consisting of three copies of the putative telomeric repeats from C. metapsilosis (lanes 1–4) or C. orthopsilosis (lanes 5–8) were used as probes (Supplemental Table S1).
Article Snippet: Pulsed-field gel electrophoresis Chromosomal DNA samples from L. elongisporus CBS5301 and C. parapsilosis CBS2194, SR23, and CBS604 T , were separated by
Techniques: Southern Blot, Pulsed-Field Gel, Electrophoresis, Labeling, Staining